Effects of Genistein on Proliferation and Cell Cycle of Salivary Adenoid Cystic Carcinoma Cells
- 期刊名字:中国癌症研究(英文版)
- 文件大小:
- 论文作者:MA Jie,WANG Jie,ZHONG Ming,WAN
- 作者单位:Department of Oral Medicine,Department of Pathology
- 更新时间:2022-04-24
- 下载次数:次
Objective: To investigate the growth inhibiting effect of tyrosine protein kinase inhibitor, genistein, on human salivary adenoid cystic carcinoma SACC-83 cell line in vitro, and its effects on the expression of CyclinB1 protein and cell cycle. Methods: Effects of genistein on the growth of SACC-83 cells in vitro were measured with MTT assay. Cell cycle was detected with flow cytometry. The expressions of CyclinB1 and Cdk1 proteins were measured with Western blot method, and the results of protein expression were quantitatively analyzed by FluorChem V2.0 software. The results were statistically analyzed by SPSS11.5 software. Results: Genistein inhibited the cell proliferation in a dose-dependant and time-dependant manner. The genistein-treated SACC-83 cells were arrested in the G2/M phase and had lower contents of CyclinB1 and Cdk1 proteins compared with the control group. Conclusion: The growth inhibiting effect of genistein on SACC-83 cells may be associated with the regulations of genistein on the CyclinB1 and Cdk1 protein expressions and the cell cycle.
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